| Autor: |
A V, Gurov, M O, Favorov, T L, Iashina, E Z, Gol'dberg, T M, Khizhniakova, V A, Anan'ev |
| Rok vydání: |
1990 |
| Předmět: |
|
| Zdroj: |
Voprosy virusologii. 35(4) |
| ISSN: |
0507-4088 |
| Popis: |
In transformation by Epstein-Barr virus of lymphocytes derived from patients with acute hepatitis B, continuous cell cultures were obtained which produced anti-HBc IgM antibodies. These cell lines underwent from 50 to 150 passages. The level of the specific immunoglobulin production was shown to have a trend to decline; however, after cloning the antibody production became more stable. The antibodies produced by the cloned lymphocyte culture could not neutralize the antigen (unlike polyclonal antibodies) which indicated a high efficacy of cloning. When the solid phase was sensitized with produced immunoglobulins, specific activity of binding of HBc antigen detectable by anti-HBc conjugate with horseradish peroxidase was demonstrated. The study of sedimentation properties of antibodies produced by transformed lymphocytes showed their sedimentation constant to be 19S. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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