| Autor: |
Ji'ai, Wang, Min, Qiao, Kangbi, Wei, Junmei, Ding, Zhongzhong, Liu, Ke-Qin, Zhang, Xiaowei, Huang |
| Rok vydání: |
2011 |
| Předmět: |
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| Zdroj: |
Journal of molecular microbiology and biotechnology. 20(4) |
| ISSN: |
1660-2412 |
| Popis: |
An Achromobacter xylosoxidans MG1 strainisolated from the effluent treatment plant of a textile and dyeing factory from Yunnan Province in China was found capable of decolorizing the malachite green dye at a high efficacy. Strain MG1 reduced 86% malachite green at the concentration of 2,000 mg/l within 1 h, representing a greater ability for decolorizing and a higher tolerance of this compound than all previously reported bacteria. Color removal was optimal at pH 6 and 38°C. Further experimental evidences demonstrated that both cytoplasmic and extracellular biodegradation contributed to the decolorization of malachite green. Nested PCR was employed to identify the candidate genes responsible for malachite green decolorization, and we identified a cytoplasmic triphenylmethane reductase gene with 100% amino acid similarity to the corresponding gene in Citrobacter sp. strain. In contrast to our expectation, the addition of metyrapone had little effect on the cytoplasmic biodegradation, suggesting that cytochrome P450 was not involved in the high-performance reduction. The extracellular biodegradation was likely attributable to the secretion of extracellular proteases and some heat-resistant compounds. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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