| Autor: |
S M, Larson, J S, Rasey, D R, Allen, N J, Nelson, Z, Grunbaum, G D, Harp, D L, Williams |
| Rok vydání: |
1980 |
| Předmět: |
|
| Zdroj: |
Journal of the National Cancer Institute. 64(1) |
| ISSN: |
0027-8874 |
| Popis: |
We studied the tumor uptake of [67Ga]citrate, [59Fe]citrate, and 125I-labeled transferrin (TF) by the in vitro growth form of EMT-6, a sarcoma-like mammary tumor of BALB/c mice. In analyzing the binding experiments, we developed a new mathematical model based on a formulation originally used to express the interaction of hormones with specific tissue receptors. The uptake of both carrier-free 67Ga and 59Fe by tumor cells was mediated by kinetically identical TF receptors. We also studied teric acid extracts of the stroma of EMT-6 tumors grown both in vivo and in vitro. Chromatography of these extracts on Sephacryl S-200 SF demonstrated that the cellular stroma contained specific TF-binding macromolecules. On the basis of these findings, we proposed the "transferrin receptor hypothesis" for the mechanism of 67Ga uptake by tumors. According to this view, a tumor-associated TF receptor is the functional unit responsible for the affinity of gallium for certain neoplasms. This receptor was also active in the uptake of iron by tumors. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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