| Autor: |
A, Rafieei, F, Riazi-Rad, M H, Alimohammadian, T, Gazori, S M R, Fatemi, M, Havaskary |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
TSitologiia i genetika. 49(2) |
| ISSN: |
0564-3783 |
| Popis: |
To facilitate the delivery of genetic material, the use of appropriate carriers such as polymers is necessary. Nanoparticles comprising of chitosan-alginate polymers were formed through pregel preparation method. Chi/Alg nanoparticles had a mean Z-Average diameter of 161.8 nm and mean zeta 29.3 mV, respectively. The ability of plasmidcomplex in preventing DNA migration showed Chi/Alg nanoparticles have great capacity to maintain plasmid. The efficiency of nanoparticles for transfection of pEGFP-N1 plasmid in the cultured HEK 293 cells was measured by flow cytometry. Cell viability assays indicated that nanoparticles had no toxic effect on HEK 293 cells after 4 or 24 h. Our suitable candidate for gene delivery would be Alg/Chi nanoparticles. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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