Binary-copolymer system base on low-density lipoprotein-coupled N-succinyl chitosan lipoic acid micelles for co-delivery MDR1 siRNA and paclitaxel, enhances antitumor effects via reducing drug

Autor:	Shu-Di, Yang, Wen-Jing, Zhu, Qiao-Ling, Zhu, Wei-Liang, Chen, Zhao-Xiang, Ren, Fang, Li, Zhi-Qiang, Yuan, Ji-Zhao, Li, Yang, Liu, Xiao-Feng, Zhou, Chun, Liu, Xue-Nong, Zhang
Rok vydání:	2015
Předmět:	Chitosan ATP Binding Cassette Transporter Subfamily B Paclitaxel Thioctic Acid Gene Transfer Techniques Mice Nude Neoplasms Experimental Xenograft Model Antitumor Assays Neoplasm Proteins Lipoproteins LDL Mice MCF-7 Cells Animals Humans Female RNA Small Interfering Micelles
Zdroj:	Journal of biomedical materials research. Part B, Applied biomaterials. 105(5)
ISSN:	1552-4981 8217-8224
Popis:	The development of effective and stable carriers of small interfering RNA (siRNA) is important for treating cancer with multidrug resistance (MDR). We developed a new gene and drug co-delivery system and checked its characteristics. Low-density lipoprotein (LDL) was coupled with N-succinyl chitosan (NSC) Lipoic acid (LA) micelles and co-delivered MDR1 siRNA and paclitaxel (PTX-siRNA/LDL-NSC-LA) to enhance antitumor effects by silencing the MDR gene of tumors (Li et al., Adv Mater 2014;26:8217-8224). In our study, we developed a new type of containing paclitaxel-loaded micelles and siRNA-loaded LDL nanoparticle. This "binary polymer" is pH and reduction dual-sensitive core-crosslinked micelles. PTX-siRNA/LDL-NSC-LA had an average particle size of (171.6 ± 6.42) nm, entrapment efficiency of (93.92 ± 1.06) %, and drug-loading amount of (12.35% ± 0.87) %. In vitro, MCF-7 cells, high expressed LDL receptor, were more sensitive to this delivery system than to taxol
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::1f041a6a17f207c479e965436588ad91 https://pubmed.ncbi.nlm.nih.gov/27008163 Zobrazit plný text záznamu Plný text