| Popis: |
Due to the problem of treating some types of burns, it is necessary to develop new drugs. For this purpose, pharmacological studies of developed gel "Xeliogel" (based on biological material with regenerating action), which accelerates the healing of superficial burns, have been developed and previously carried out.The aim of this work was to establish the histological changes of the burn wound in the dynamics and after the experimental thermal injury and in the conditions of application of the gel "Xeliogel".The experiments were performed on mature Wistar rats of both sexes weighing 250-260 g, randomly divided equally into four groups: 1 - group of intact animals; 2 - control pathology group; 3 - group for the treatment of which used the developed gel "Xeliogel" and 4 - group of animals with the comparison drug "Solcoseryl" (Legacy Pharmaceuticals Switzerland GmbH, Switzerland). Histological indicators of the effect of gels were recorded 3 times: on the 3rd (stage of burn shock), 7th (stage of early toxemia) and 14th (stage of late toxemia) days of the experiment. Examination of micropreparations was performed on a Nicon Eclipse CI-E microscope. Microscopy of microscopic images was performed using a Sigeta M3CMOS 14000 camcorder and Toup View software on a personal computer.During using the developed gel "Xeliogel" it is established that on the 3rd day of the experiment the wound surface is covered with a crust, which is formed by plasma proteins and with destroyed elements of blood. On day 7 after the experimental thermal injury, both "Xeliogel" gel and "Solcoseryl" gel were found to show that the skin defect area was also covered with a film, the main components of which were destroyed blood cells and fibrinous mass. When examining the area of the defect on the 14th day of the experiment with the use of the comparison drug "Solcoseryl" gel, wounds healing covered with an epithelial layer with a clear-layered structure was observed.The histological evaluation of the use of "Xeliogel" gel established that the developed gel provides healing of the wound defect on the 14th day of the experiment. There is a well-defined marginal regeneration of the epidermis, the formation of the basement membrane, the restoration of the papillary layer of the dermis and capillary system. |
