| Popis: |
The inhibition of RNA synthesis by DRB (5,6-dichloro-1-beta-D-ribofuranosyl benzimidazole) differs in different regions of D. melanogaster polytene chromosomes. In the majority of regions RNA synthesis is greatly inhibited, but in certain regions, including different development stage-specific puffs, it is still on. A case of transcription initiation in the heat shock puffs was also found. alpha-Amanitin considerably reduced RNA transcription in the majority of nuclei but some part of the nuclei are not affected at all. A combined action of alpha-amanitin and DRB exerts a considerably higher inhibitory effect. |
