Cloning and characterization of the gene coding for the human acrosomal protein SP-10
| Autor: | R M, Wright, A K, Suri, B, Kornreich, C J, Flickinger, J C, Herr |
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| Rok vydání: | 1993 |
| Předmět: |
Male
Base Sequence Transcription Genetic Molecular Sequence Data Membrane Proteins Nucleic Acid Hybridization Sequence Homology Exons Polymerase Chain Reaction Alternative Splicing Testis Humans Female Amino Acid Sequence RNA Messenger Antigens Cloning Molecular DNA Probes Gonadal Steroid Hormones Acrosome Sequence Analysis |
| Zdroj: | Biology of reproduction. 49(2) |
| ISSN: | 0006-3363 |
| Popis: | SP-10 is a human intra-acrosomal protein that is first detected in the developing acrosome of round spermatids and is observed associated with the acrosomal membranes and matrix of mature sperm. Recombinant SP-10 is currently being tested as a contraceptive vaccine immunogen on the basis of its tissue specificity as well as functional assays indicating that anti-SP-10 antisera inhibit sperm-egg interactions. In the present study, structural characterization of the human SP-10 gene was undertaken to investigate the expression of the gene and to identify possible functions of the SP-10 protein within the acrosome. Genomic blots indicate that SP-10 is encoded by a single-copy gene. Mapping and sequencing of the 8-kb SP-10 gene show that the SP-10 mRNA consists of exons of 119, 487, 113, and 390 bp with each exon coding for a distinct structural domain within the SP-10 protein. An in-frame alternatively spliced form of the SP-10 mRNA, identified during SP-10 cDNA characterization, employs the same 3' splice site as the 487-bp exon and a unique 5' splice site within the 487-bp exon. Previous comparisons of human, baboon, and macaque SP-10 cDNA sequences indicated that the baboon and macaque cDNAs contained 60 bp (20 aa) not present in the human SP-10 cDNA. Analysis of the human SP-10 genomic sequence suggests that a 60-bp deletion may have occurred during the evolution of the human SP-10 gene by homologous recombination.(ABSTRACT TRUNCATED AT 250 WORDS) |
| Databáze: | OpenAIRE |
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