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This study investigated the effects of salvianolic acid B (Sal B), a major bioactive component of the Chinese medicine salvia miltiorrhiza, on osteogenic differentiation of human periodontal ligament cells (hPDLCs).Third passage PDLCs were used in this experiment. Methyl thiazolyl tetrazolium (MTT) method was employed to observe the effects of different Sal B concentrations on proliferation activity of hPDLCs. Alkaline phosphatase (ALP) activity and mineralization capability were measured, and mRNA expression of osteocalcin (OCN) was detected to investigate the effects of Sal B on osteogenesis of hPDLCs.Sal B did not influence the viability of hPDLCs. The ALP activity and OCN mRNA expression levels of hPDLCs were both significantly improved (P0.05) under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L⁻¹) compared with those in OIM group. Moreover, the number of mineralized nodules formed by hPDLCs were considerably higher under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L⁻¹) than that in the OIM group.Appropriate Sal B concentration can improve the osteogenic differentiation of hPDLCs.目的 探讨中药丹参主要活性成分丹酚酸B（Sal B）对人牙周膜细胞（hPDLCs）成骨分化的影响。方法 取第3代hPDLCs进行实验，运用甲基噻唑基四唑（MTT）法检测不同浓度丹酚酸B对hPDLCs增殖活性影响，通过检测碱性磷酸酶（ALP）活性、矿化结节染色、骨钙素（OCN）mRNA表达来探讨丹酚酸B对hPDLCs成骨分化的影响。结果 丹酚酸B对hPDLCs增殖活性无影响。当丹酚酸B浓度为0.5、1、5 μmol·L⁻¹时均能增加hPDLCs的ALP活性和OCN mRNA表达，与OIM组比较差异有统计学意义（P0.05）；且当丹酚酸B浓度为0.5、1、5 μmol·L⁻¹时hPDLCs形成矿化结节数量明显高于OIM组。结论 适宜浓度的丹酚酸B能有效促进hPDLCs成骨分化。. |
