MMP-19: cellular localization of a novel metalloproteinase within normal breast tissue and mammary gland tumours
| Autor: | V, Djonov, K, Högger, R, Sedlacek, J, Laissue, A, Draeger |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Adolescent Cell Culture Techniques Down-Regulation Metalloendopeptidases Breast Neoplasms Epithelial Cells Muscle Smooth Middle Aged Immunoenzyme Techniques Matrix Metalloproteinases Secreted Disease Progression Humans Matrix Metalloproteinase 2 Female Neoplasm Invasiveness Breast Endothelium Vascular Aged |
| Zdroj: | The Journal of pathology. 195(2) |
| ISSN: | 0022-3417 |
| Popis: | Matrix metalloproteinases (MMPs) are instrumental in promoting and facilitating the spread of malignant diseases and in the de novo formation of blood vessels. This study has mapped the immunoreactivity of a novel, angiogenesis-related metalloproteinase--MMP-19--in normal breast tissue and in benign and malignant breast lesions and compared this pattern of expression with that of MMP-2. In the normal resting mammary gland, MMP-19 was strongly expressed in the myoepithelial layer of the ductal system; the alveolar and ductal epithelia displayed considerable, but lobule-specific, variations in labelling intensity. MMP-19 was also present within the smooth muscle and endothelial layers of large and medium-sized blood vessels, as well as within capillary walls. In benign lesions, all tumour cells and their surrounding vasculature were uniformly and strongly immunoreactive for MMP-19. Progression towards an invasive phenotype and neoplastic dedifferentiation led to the disappearance of MMP-19 from tumour cells and blood vessels and a concomitant rise in the levels of MMP-2. In vitro experiments conducted with isolated smooth muscle cells cultivated on a solid substratum, or within the interstices of a collagen matrix, indicated that the expression of MMP-19 is influenced by the architecture of the surrounding extracellular matrix. |
| Databáze: | OpenAIRE |
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