Cloning of equine interleukin 1 receptor antagonist and determination of its full-length cDNA sequence
| Autor: | R D, Howard, C W, McIlwraith, G W, Trotter, J K, Nyborg |
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| Rok vydání: | 1998 |
| Předmět: |
DNA
Complementary Base Sequence Sequence Homology Amino Acid Sialoglycoproteins Molecular Sequence Data Receptors Interleukin-1 Polymerase Chain Reaction Monocytes Recombinant Proteins Rats Interleukin 1 Receptor Antagonist Protein Mice Animals Humans Amino Acid Sequence Horses Rabbits Cloning Molecular DNA Probes Sequence Alignment Gene Library |
| Zdroj: | American journal of veterinary research. 59(6) |
| ISSN: | 0002-9645 |
| Popis: | To clone equine interleukin 1 receptor antagonist (IL-1ra) and determine its full-length cDNA sequence.A cDNA library derived from lipopolysaccharide-stimulated equine monocytes was screened by means of plaque hybridization to radiolabeled equine IL-1ra DNA probes generated by means of the polymerase chain reaction. The cDNA nucleotide sequence for equine IL-1ra was determined by use of the dideoxy chain termination technique, analyzed by use of computer software for sequence characteristics, and compared with sequences reported for IL-1ra of other species.The cDNA of equine IL-1ra was 1,614 base pairs in length with an ORF encoding a peptide of 177 amino acids with a predicted molecular mass of 20.427 kd. Similarity between the amino acid sequence of equine IL-1ra and sequences for human, murine, rat, and lapine IL-1ra was 76%. Similarity between sequence for equine IL-1ra and sequences for equine interleukin-1 alpha and equine interleukin-1 beta were 22.6 and 24.6%, respectively.Comparison of the sequence for equine IL-1ra with sequences for IL-1ra of other species indicated a high degree of conservation.Results establish a basis for studying the roles of interleukin-1 in healthy and diseased joints in horses. |
| Databáze: | OpenAIRE |
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