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The present study investigates the effects of perfluorooctanoic acid (PFOA) exposure on reproductive toxicity and uterine apoptosis in pregnant mice. Sixty pregnant mice were randomly divided into 6 groups (groups A, B, C, D, E and F). In control group (A), the mice received distilled water at 10 mg/kg body weight per day on gestation days (GD) 1 to 17. The mice in group B, C, D, E and F were treated with PFOA solution at 1, 5, 10, 20 and 40 mg/kg body weight respectively from GD1 to GD17. The mice were sacrificed on GD18. The distribution and expression of Fas, FasL, Bcl-2, Bax, and Caspase-3 in uterine cells were detected by immunohistochemistry. The apoptosis of uterine cells was detected by TdT-mediated dUTP Nick-End Labeling (TUNEL). Results showed that the expression of Fas, FasL, and Caspase 3 in uterus increased significantly after PFOA was applied. The expression of Bcl-2 was decreased significantly and the expression of Bax was increased significantly. The ratio of Bcl-2/Bax decreased significantly compared with the control group (P |
