| Autor: |
J C, Drake, C J, Allegra, G A, Curt, B A, Chabner |
| Rok vydání: |
1985 |
| Předmět: |
|
| Zdroj: |
Cancer treatment reports. 69(6) |
| ISSN: |
0361-5960 |
| Popis: |
A competitive protein-binding assay has been developed for trimetrexate (TMTX) based on the tight binding of this drug to human dihydrofolate reductase (tetrahydrofolate dehydrogenase). In this assay, TMTX competes with 3H-methotrexate for binding to the enzyme. Free drug is separated from that bound to reductase by adsorption with dextran-albumin-coated charcoal. TMTX is measurable over a range from 2 X 10(-9) to 5 X 10(-8) M in plasma, with a coefficient of variation of less than 10%. Measurements of TMTX in plasma, cerebrospinal fluid, and urine agreed closely with parallel determinations in aqueous solutions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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