Molecular Imaging of Inflammation in a Mouse Model of Atherosclerosis Using a Zirconium-89-Labeled Probe
| Autor: | Mona, Ahmed, Tetyana, Tegnebratt, Thuy A, Tran, Li, Lu, Peter, Damberg, Anton, Gisterå, Laura, Tarnawski, Dianna, Bone, Ulf, Hedin, Per, Eriksson, Staffan, Holmin, Björn, Gustafsson, Kenneth, Caidahl |
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| Rok vydání: | 2020 |
| Předmět: |
positron emission tomography
Mice Knockout ApoE zirconium Serum Albumin Human Fluorodeoxyglucose F18 Animals Humans Tissue Distribution Aorta Original Research Radioisotopes Macrophages Maleates Atherosclerosis molecular imaging Magnetic Resonance Imaging Plaque Atherosclerotic body regions Mice Inbred C57BL Disease Models Animal human serum albumin Isotope Labeling Molecular Probes Positron-Emission Tomography embryonic structures Autoradiography Female Radiopharmaceuticals |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Background Beyond clinical atherosclerosis imaging of vessel stenosis and plaque morphology, early detection of inflamed atherosclerotic lesions by molecular imaging could improve risk assessment and clinical management in high-risk patients. To identify inflamed atherosclerotic lesions by molecular imaging in vivo, we studied the specificity of our radiotracer based on maleylated (Mal) human serum albumin (HSA), which targets key features of unstable atherosclerotic lesions. Materials and Methods Mal-HSA was radiolabeled with a positron-emitting metal ion, zirconium-89 (89Zr4+). The targeting potential of this probe was compared with unspecific 89Zr-HSA and 18F-FDG in an experimental model of atherosclerosis (Apoe–/– mice, n=22), and compared with wild-type (WT) mice (C57BL/6J, n=21) as controls. Results PET/MRI, gamma counter measurements, and autoradiography showed the accumulation of 89Zr-Mal-HSA in the atherosclerotic lesions of Apoe–/– mice. The maximum standardized uptake values (SUVmax) for 89Zr-Mal-HSA at 16 and 20 weeks were 26% and 20% higher (P |
| Databáze: | OpenAIRE |
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