| Autor: |
Ewa, Bocian, Justyna, Kasprzycka, Krystyna, Jakubów-Durska, Alicja, Łuszczek, Joanna, Bernaciak |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Ginekologia polska. 82(9) |
| ISSN: |
0017-0011 |
| Popis: |
The study was aimed to determine diagnostic application of MLPA for rapid prenatal identification of chromosome 13, 18, 21 and X and Y aneuploidies.409 amniotic fluid samples from amniocentesis for fetal karyotyping were studied. DNA was isolated using the QIAmp DNA Blood Midi Kit (348 samples) or through proteinase K treatment (61 samples). SALSA MLPA P095 probes (mrc-Holland) were used to detect aneuploidyIn 324 studies (79.2%) diagnostic results were obtained. Chromosomal aberrations were found in 16 cases (4.9%). These results were concordant with standard karyotype. In 3 cases (0.92%) false negative results were found but all abnormalities were undetectable with MLPA.MLPA is a reliable method of rapid prenatal detection of aneuploidy |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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