Altered microglial phagocytosis in GPR34-deficient mice
| Autor: | Julia, Preissler, Antje, Grosche, Vera, Lede, Diana, Le Duc, Katja, Krügel, Vitali, Matyash, Frank, Szulzewsky, Sonja, Kallendrusch, Kerstin, Immig, Helmut, Kettenmann, Ingo, Bechmann, Torsten, Schöneberg, Angela, Schulz |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
CD11b Antigen
Tumor Necrosis Factor-alpha Green Fluorescent Proteins CX3C Chemokine Receptor 1 Brain Mice Transgenic In Vitro Techniques Flow Cytometry Retina Mice Inbred C57BL Mice Organ Culture Techniques Gene Expression Regulation Phagocytosis Receptors Lysophospholipid Cell Movement Animals Receptors Chemokine Microglia |
| Zdroj: | Glia. 63(2) |
| ISSN: | 1098-1136 |
| Popis: | GPR34 is a Gi/o protein-coupled receptor (GPCR) of the nucleotide receptor P2Y12 -like group. This receptor is highly expressed in microglia, however, the functional relevance of GPR34 in these glial cells is unknown. Previous results suggested an impaired immune response in GPR34-deficient mice infected with Cryptococcus neoformans. Here we show that GPR34 deficiency results in morphological changes in retinal and cortical microglia. RNA sequencing analysis of microglia revealed a number of differentially expressed transcripts involved in cell motility and phagocytosis. We found no differences in microglial motility after entorhinal cortex lesion and in response to laser lesion. However, GPR34-deficient microglia showed reduced phagocytosis activity in both retina and acutely isolated cortical slices. Our study identifies GPR34 as an important signaling component controlling microglial function, morphology and phagocytosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text