Recombinant Gluconacetobacter diazotrophicus containing Cry1Ac gene codes for 130-kDa toxin protein
| Autor: | M, Subashini, A, Moushumi Priya, B, Sundarakrishnan, S, Jayachandran |
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| Rok vydání: | 2011 |
| Předmět: |
Recombination
Genetic Bacillus thuringiensis Toxins Immunoblotting Bacillus thuringiensis India Polymerase Chain Reaction Saccharum Endotoxins Lepidoptera Gluconacetobacter Hemolysin Proteins Bacterial Proteins Larva Nitrogen Fixation Nitrogenase Microscopy Electron Scanning Animals Electrophoresis Polyacrylamide Gel Pest Control Biological |
| Zdroj: | Journal of molecular microbiology and biotechnology. 20(4) |
| ISSN: | 1660-2412 |
| Popis: | Recombinant Gluconacetobacter diazotrophicus containing Cry1Ac gene from Bacillus thuringiensis var. kurstaki borne on pKT230, shuttle vector, was generated. PCR amplification of Cry1Ac gene present in recombinant G. diazotrophicus yielded a 278-bp DNA product. The nitrogenase assay has revealed that the recombinant G. diazotrophicus in sugarcane stem produced similar levels of nitrogenase compared to wild-type G. diazotrophicus. The presence of 130-kDa protein in apoplastic fluid from sugarcane stem harvested from pots inoculated with recombinant G. diazotrophicus shows that the translocated G. diazotrophicus produces 130-kDa protein which is recognized by the hyperimmune antiserum raised against 130-kDa protein. The first instar Eldana saccharina neonate larvae that fed on artificial medium containing recombinant G. diazotrophicus died within 72 h after incubation. |
| Databáze: | OpenAIRE |
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