| Autor: |
Jia, Liu, Ya-Jun, Liang, Pei-Ling, Ren, Thomas, Gaj |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1867 |
| ISSN: |
1940-6029 |
| Popis: |
Genome-editing technologies have revolutionized the biomedical sciences by providing researchers with the ability to quickly and efficiently modify genes. While programmable nucleases can be introduced into cells using a variety of techniques, their delivery as purified proteins is an effective approach for limiting off-target effects. Here, we describe step-by-step procedures for manufacturing and delivering genome-modifying proteins-including Cas9 ribonucleoproteins (RNPs) and TALE and zinc-finger nucleases-into mammalian cells. Protocols for combining Cas9 RNP with naturally recombinogenic adeno-associated virus (AAV) donor vectors for the seamless insertion of transgenes by homology-directed genome editing are also provided. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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