| Autor: |
Ji-Ping, Xia, Xiu-Zu, Song, Wei-Ling, Sun, Yan-Hua, Li, Jian, Kang, Wen-Qi, Chen, Zhi-Gang, Bi |
| Rok vydání: |
2007 |
| Předmět: |
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| Zdroj: |
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae. 29(2) |
| ISSN: |
1000-503X |
| Popis: |
To explore the effects of antisense epidermal growth factor receptor (EGF-R) oligodeoxynucleotides on ultraviolet-induced c-jun activity of keratinocytes after EGF-R oligodeoxynucleotides transfect to HaCaT in vitro.c-jun DNA binding activity after ultraviolet-B (UVB) irradiation and EGF-R oligodeoxynucleotides transfection were determined with a highly sensitive and specific colorimetric method. After EGF-R oligodeoxynucleotides transfection, the mRNA level of EGF-R was detected by reverse transcription polymerase chain reaction method.Compared with control groups, c-jun activity increased significantly in UVB (10, 20, 30 mJ/cm2) irradiation groups (P0.05). EGF-R mRNA and c-jun activities induced by UVB were inhibited after the keratinocytes were transfected with EGF-R antisense oligodeoxynucleotides at 2, 4 and 8 microg/ml concentrations (P0.01).The ultraviolet-induced c-jun activity of keratinocytes can be mediated by EGF-R and inhibited by EGF-R antisense oligodeoxynucleotides, which is transfected to keratinocytes and mediated by lipofectamine. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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