Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis

Autor: Chloe, Potter, Didier, Hodzic
Rok vydání: 2018
Předmět:
Zdroj: Methods in molecular biology (Clifton, N.J.). 1840
ISSN: 1940-6029
Popis: The biochemical characterization of proteins most often require their identification by immunoblotting. Whereas SDS-PAGE provides satisfactory results for most proteins, the identification of larger proteins requires alternative methods to ensure their separation and complete transfer onto nitrocellulose membranes. Here, we describe the application of vertical agarose gel electrophoresis to identify large isoforms of nesprin-1 and nesprin-2.
Databáze: OpenAIRE