| Autor: |
B J, Zeng, P, Ji, Z J, Sun, J L, Wu, A Q, Shang, W W, Quan, D, Li |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]. 55(5) |
| ISSN: |
0253-9624 |
| Popis: |
To explore the expression of p62 protein in lung adenocarcinoma (LUAD). In this study, a cross-sectional study was adopted. From December 2011 to May 2013, 60 patients with lung adenocarcinoma who were diagnosed and treated in Tongji Hospital of Tongji University, Shanghai were selected for paraffin embedding and tissue chip preparation, and immunohistochemistry (IHC) technology was used to detect the expression of p62 in lung adenocarcinoma patients' cancer tissues and adjacent tissues, and analyze the relationship between p62 expression and the clinicopathological characteristics and survival prognosis of patients with lung adenocarcinoma; at the same time, 6 cases of lung adenocarcinoma were selected by random sampling cancer tissues and adjacent tissues were detected by Western Blot (WB) to detect p62 protein and analyzed by gray value. Preoperative examination specimens of inpatients with lung adenocarcinoma diagnosed from April 2018 to early October 2019, and plasma specimens of healthy subjects were collected, and enzyme linked immunosorbent assay (ELISA) was used to detect lung adenocarcinoma patients and healthy patients. The expression of p62 in the plasma of the subjects was statistically analyzed using SPSS 22.0 software. The results of IHC showed that the positive expression rate of p62 in cancer tissues was significantly higher than that in adjacent tissues, and the difference was statistically significant (探讨p62蛋白在肺腺癌（lung adenocarcinoma，LUAD）中的表达情况。采用横断面研究，选择2011年12月至2013年5月上海市同济大学附属同济医院诊治的60例肺腺癌患者组织标本进行石蜡包埋和组织芯片制作，采用免疫组织化学技术（immunohistochemistry，IHC）检测肺腺癌患者癌组织和癌旁组织中p62的表达，分析p62表达与肺腺癌患者临床病理特征及生存预后的关系；同时以随机抽样的方式选取6例肺腺癌癌组织和癌旁组织通过蛋白免疫印迹（Western Blot，WB）检测p62蛋白并进行灰度值分析。收集2018年4月至2019年10月初诊的肺腺癌住院患者术前检查标本，以及健康体检者血浆标本，酶联免疫吸附法（enzyme linked immunosorbent assay，ELISA）分别检测肺腺癌患者和健康体检者血浆中p62的表达情况。IHC结果显示癌组织中的p62阳性表达率显著高于癌旁组织，差异有统计学意义（ |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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