[The age-related specificity of site-oriented immunity in respiratory-syncytial virus infection]
| Autor: | V Z, Krivitskaia, A A, Sominina, G P, Vlasov, V F, Sukhovetskaia, A K, Golovanova |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Aging Adolescent Infant Enzyme-Linked Immunosorbent Assay Respiratory Syncytial Virus Infections Antibodies Viral Protein Structure Tertiary Epitopes Viral Proteins Species Specificity Neutralization Tests Child Preschool Respiratory Syncytial Virus Human Antibody Formation Humans Child Peptides Antigens Viral Viral Fusion Proteins |
| Zdroj: | Voprosy virusologii. 49(4) |
| ISSN: | 0507-4088 |
| Popis: | ELISA test systems were designed on the basis of synthetic peptides (SP) simulating the primary structure of functionally significant epitopes of the respiratory and cyncyntial virus (RCV) F-protein for the purpose of investigating the structure and age-related peculiarities of humoral immunity in respect to separate epitopes of RCV F-protein. One of them (221-232) simulates a part of RCV "virus-neutralizing domain" and another one (479-491) is highly important for the fusion mechanisms. New SP-based ELISA were used to examine pair sera in 159 patients with documented RCV infection including children, aged up to 3 years and 3 to 15, and adults. The activity of anti-RCV antibodies to SP was found to be significantly lower in children aged up to 3 years versus the older children and especially versus the adults. The virus neutralizing and, to a greater extent, fusion-inhibiting activities of antibodies were increasing with age, which collated with the results of detecting the antibodies to SP by immune-enzyme assay. The results testify to synchronism of formation of antibodies to different epitopes of the RCV F-protein. The shaping-up of antibodies with the above SP could denote the protective properties of humoral immunity, which justifies the use of the SP-based ELISA in its analysis, especially, in babies as well as in different-type immunodeficiency and immunopathology conditions. |
| Databáze: | OpenAIRE |
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