| Autor: |
Kathleen A, Lesich, Courtney B, Kelsch, Kristen L, Ponichter, Benjamin J, Dionne, Loan, Dang, Charles B, Lindemann |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Biology of reproduction. 86(4) |
| ISSN: |
1529-7268 |
| Popis: |
Triton X-100-extracted mouse sperm treated with 0.1 mM ATP and 1.0 mM Ca(2+) exhibit an extremely coiled configuration that has been previously described as a curlicue. Sperm in the curlicue configuration exhibit a monotonically curved flagellum where the shear angle of the flagellum can reach a value as high as 14 radians at the flagellar tip. We utilized this strong reaction to Ca(2+) to elucidate the mechanism of the calcium response. The disintegration of the axoneme was facilitated by the use of an extraction procedure that removed the mitochondrial sheath without eliminating the calcium response. The order of emergence of the doublet microtubule outer dense fiber complexes was observed in the presence and absence of added Ca(2+). The identity of the emergent elements was confirmed by transmission electron microscopy. Ca(2+) altered the order of emergence of internal axoneme elements to favor the appearance of the elements of the 9-1-2 side of the axoneme. These elements are propelled baseward by the action of dyneins on doublets 1 and 2. It was also possible to establish that the motive force for maintaining the curlicue configuration is dynein-based. The curlicues were relaxed by inhibition with 50 μM NaVO(3) and were reestablished by disinhibiting the vanadate with 2.5 mM catechol. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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