| Autor: |
J, Bas, M, Mestre, J M, Griñó, E, Massip, A M, Castelao, A, Romeu, L, González, A, Valls, E, Buendía |
| Rok vydání: |
1993 |
| Předmět: |
|
| Zdroj: |
Allergologia et immunopathologia. 21(4) |
| ISSN: |
0301-0546 |
| Popis: |
In order to assess the immune mechanisms triggered by an immunosuppressive regimen consisting of prophylactic antilymphocyte globulin plus low-dose cyclosporine A and steroids, we studied the short-term evolution of both, anti donor in vitro alloresponse and peripheral blood T cell subsets in 21 recipients of a cadaveric kidney allograft. Spleen cells from cadaveric donors and peripheral blood lymphocytes from the respective recipients pretransplant (pre-Tx), at three and six months posttransplant (post-Tx) were obtained to perform one-way mixed lymphocyte cultures and flow cytometry analysis of lymphocyte subsets. The results indicated the development of donor-specific mixed lymphocyte culture (MLC) hyporesponsiveness as early as three months post-Tx, paralleled by a decrease in CD4+CD29+ helper-inducer cells and by an increase in CD8+CD45RA+ suppressor lymphocytes in peripheral blood. These changes were reflected in a very good clinical outcome of the patients. The present results further suggest that suppression of the immune system just before transplantation is a suitable method to induce early specific hyporesponsiveness to the allograft. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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