| Autor: |
Aaron, Ang, Eric, Ouellet, Karen C, Cheung, Charles, Haynes |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1768 |
| ISSN: |
1940-6029 |
| Popis: |
In addition to its growing use in detecting and quantifying genes and larger genomic events, the partitioning used in digital PCR can serve as a powerful tool for high-fidelity amplification of synthetic combinatorial libraries of single-stranded DNA. Sequence-diverse libraries of this type are used as a basis for selecting tight-binding aptamers against a specific target. Here we provide a detailed description of the Hi-Fi SELEX protocol for rapid and efficient DNA aptamer selection. As part of that methodology, we describe how Hi-Fi SELEX gains advantages over other aptamer selection methods in part through the use of the massive partitioning capability of digital PCR. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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