Identification of proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1 in the fission yeast Schizosaccharomyces pombe

Autor:	Cipakova, Ingrid, Jurcik, Matus, Rubintova, Veronika, Borbova, Marianna, Mikolaskova, Barbora, Jurcik, Jan, Bellova, Jana, Barath, Peter, Gregan, Juraj, Cipak, Lubos
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Extra View Nrl1 RNA Splicing NRDE-2 homologous recombination fission yeast Spliceosome Schizosaccharomyces Spliceosomes R-loop RNA Splicing Factors Schizosaccharomyces pombe Proteins R-Loop Structures RNA Helicases
Zdroj:	Cell Cycle
ISSN:	1551-4005 1538-4101
Popis:	The spliceosome is a complex molecular machine assembled from many components, which catalyzes the removal of introns from mRNA precursors. Our previous study revealed that the Nrl1 (NRDE-2 like 1) protein associates with spliceosome proteins and regulates pre-mRNA splicing and homologous recombination-dependent R-loop formation in the fission yeast Schizosaccharomyces pombe. Here, we identify proteins associated with splicing factors Ntr1, Ntr2, Brr2 and Gpl1, a poorly characterized G-patch domain-containing protein required for efficient splicing. This work provides new evidence that Nrl1 and splicing factors physically interact and reveals additional insights into the protein interaction network of the spliceosome. We discuss implications of these findings in the light of recent progress in our understanding of how Nrl1 and splicing factors ensure genome stability.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::1c66f909ad73d79ac8d747776d6ee07d http://europepmc.org/articles/PMC6619935 Zobrazit plný text záznamu