| Autor: |
Ewa, Obłak, Tadeusz M, Lachowicz, Katarzyna, Waszkiewicz, Janina, Kuduk-Jaworska |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
Cellularmolecular biology letters. 8(4) |
| ISSN: |
1425-8153 |
| Popis: |
A series of cytotoxic neutral dicarboxylatoplatinum(II) complexes containing D(+), L(-) or DL-malate dianion and ethylenediamine or 1-ethylimidazole as ligands were examined using ATPase activity assays and the proton extrusion test. ATPase activity assays in vitro on plasma membrane H+-ATPase and on mitochondrial ATPase were carried out. The concentrations of compounds inhibiting enzyme activity to 50 per cent (J50) was determined. The new platinum complexes showed a stronger level of inhibition of both ATPases than the reference carboplatin; this inhibitory activity is related to a stereoisomeric form of anionic platinum ligands. ATPase inhibition in vivo was tested by glucose-stimulated proton extrusion and the influence of platinum compounds on this process in yeast cells was determined. Significant differences in activity levels were observed between those complexes with 1-ethylimidazole and those with ethylenediamine. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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