[Study on multipotential differentiation of human bone marrow mesenchymal stem cells in vitro]

Autor: Ying, Chang, Xin, Qi, Li-sha, Bu, Zhong-xin, Xu
Rok vydání: 2005
Předmět:
Zdroj: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue. 17(2)
ISSN: 1003-0603
Popis: To study the capability of human bone marrow mesenchymal stem cells (MSCs) to differentiate into adipocyte, osteoblasts, and neurocytes under different experimental conditions in vitro.The induced human bone marrow MSCs were examined by cytochemistry staining and immunohistochemistry staining.Induced human bone marrow MSCs formed alkaline phosphatase-positive aggregates and Von Kossa stain-positive nodules under the condition of osteogenic differentiation. Under the condition of adipogenic differentiation, the isolated human bone marrow MSCs formed oil red-O-positive cells. Immunohistochemistry results showed that differentiated human bone marrow MSCs expressed neuron-specific enolase (NSE) and neurofilament (NF-M).Human bone marrow MSCs have the capability of multipotential differentiation in vitro.
Databáze: OpenAIRE