| Autor: |
J D, Lee, T M, Sievers, M, Skotzko, C F, Chandler, D L, Morton, W H, McBride, J S, Economou |
| Rok vydání: |
1992 |
| Předmět: |
|
| Zdroj: |
Lymphokine and cytokine research. 11(3) |
| ISSN: |
1056-5477 |
| Popis: |
We examined the expression of interleukin-6 (IL-6) by 12 established human melanoma cell lines. Two constitutively produced low levels of IL-6 protein, as measured by enzyme-linked immunosorbent assay. Cells from these two lines, as well as those from two non-IL-6-producing cell lines, contained IL-6-specific mRNA as demonstrated by Northern hybridization. Treatment of the two IL-6-producing melanoma cell lines with interleukin-1 beta, tumor necrosis factor-alpha, or phorbol myristate acetate caused a marked increase in IL-6 production. These induction signals failed to stimulate IL-6 production in the nonproducing cells, even those that expressed IL-6 mRNA. IL-6 did not appear to act as an autocrine growth factor since the addition of exogenous human recombinant IL-6 or polyclonal anti-IL-6 antibody did not alter cellular proliferation. The production of this multifunctional cytokine by tumors may play a role in tumor-host interactions and this should be recognized in the design of biologic therapy trials. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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