| Autor: |
Madhan, Masilamani, Mariona, Pascal, Hugh A, Sampson |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1592 |
| ISSN: |
1940-6029 |
| Popis: |
Characterization of allergen-specific T cells is critical to understand their contribution to disease pathogenesis. The identification of immunodominant T-cell epitopes is crucial for development of T-cell-based vaccines. Peptide-specific T-cell proliferation studies are usually performed in a library of short synthetic peptides (15mer or 20mer) with 3 or 5 offset spanning the entire length of the allergen. T-cell peptide epitopes lack the primary and tertiary structure of the native protein to cross-link IgE, but retain the ability to stimulate T cells. The peptides sequences can also be obtained either by in silico approaches and in vitro binding assays. The efficacy of T-cell epitope-based peptide immunotherapy has been proven in certain allergies. The present methodology describes T-cell proliferation assays using whole blood sample from allergic subjects. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
