Capillary zone electrophoresis for the study of the binding of antithrombin to low-affinity heparin

Autor:	K, Gunnarsson, L, Valtcheva, S, Hjertén
Rok vydání:	1998
Předmět:	Binding Sites Heparin Swine Animals Electrophoresis Capillary Antithrombins
Zdroj:	Glycoconjugate journal. 14(7)
ISSN:	0282-0080
Popis:	When low-affinity interactions between glycosaminoglycans and precious proteins are studied, it is imperative to design an experimental set-up that consumes as little material as possible. To evaluate the applicability of the CZE technique to this problem, we explored the interaction between antithrombin and low-affinity heparin. In a series of CZE experiments we demonstrated that the mobility of antithrombin increases gradually as increased concentrations of low-affinity heparin were added to the electrolyte. The results were, as expected, consistent with the general algorithm for monovalent binding. The binding constant was estimated at 20+/-6 microM in excellent agreement with the value reported in the literature.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::1b7cb6dab17693d0f5634d9682f84ce6 https://pubmed.ncbi.nlm.nih.gov/9511991 Zobrazit plný text záznamu Full text from SpringerLink