Exploring the evolution and epidemiology of European CC1-MRSA-IV: tracking a multidrug-resistant community-associated meticillin-resistant
| Autor: | Megan R, Earls, Eike J, Steinig, Stefan, Monecke, José A, Samaniego Castruita, Alexandra, Simbeck, Wulf, Schneider-Brachert, Teodora, Vremerǎ, Olivia S, Dorneanu, Igor, Loncaric, Michèle, Bes, Alicia, Lacoma, Cristina, Prat Aymerich, Ulrich, Wernery, Marc, Armengol-Porta, Anita, Blomfeldt, Sebastian, Duchene, Mette D, Bartels, Ralf, Ehricht, David C, Coleman |
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| Rok vydání: | 2021 |
| Předmět: |
Methicillin-Resistant Staphylococcus aureus
Cross Infection Whole Genome Sequencing CA-MRSA transmission phylogenomics Microbial Sensitivity Tests Pathogens and Epidemiology Staphylococcal Infections European CC1-MRSA-IV clone Anti-Bacterial Agents Community-Acquired Infections Europe Evolution Molecular Drug Resistance Multiple Bacterial evolution Animals Humans epidemiology Genome Bacterial Phylogeny Research Articles |
| Zdroj: | Microbial Genomics |
| ISSN: | 2057-5858 |
| Popis: | This study investigated the evolution and epidemiology of the community-associated and multidrug-resistant Staphylococcus aureus clone European CC1-MRSA-IV. Whole-genome sequences were obtained for 194 European CC1-MRSA-IV isolates (189 of human and 5 of animal origin) from 12 countries, and 10 meticillin-susceptible precursors (from North-Eastern Romania; all of human origin) of the clone. Phylogenetic analysis was performed using a maximum-likelihood approach, a time-measured phylogeny was reconstructed using Bayesian analysis, and in silico microarray genotyping was performed to identify resistance, virulence-associated and SCCmec (staphylococcal cassette chromosome mec) genes. Isolates were typically sequence type 1 (190/204) and spa type t127 (183/204). Bayesian analysis indicated that European CC1-MRSA-IV emerged in approximately 1995 before undergoing rapid expansion in the late 1990s and 2000s, while spreading throughout Europe and into the Middle East. Phylogenetic analysis revealed an unstructured meticillin-resistant S. aureus (MRSA) population, lacking significant geographical or temporal clusters. The MRSA were genotypically multidrug-resistant, consistently encoded seh, and intermittently (34/194) encoded an undisrupted hlb gene with concomitant absence of the lysogenic phage-encoded genes sak and scn. All MRSA also harboured a characteristic ~5350 nt insertion in SCCmec adjacent to orfX. Detailed demographic data from Denmark showed that there, the clone is typically (25/35) found in the community, and often (10/35) among individuals with links to South-Eastern Europe. This study elucidated the evolution and epidemiology of European CC1-MRSA-IV, which emerged from a meticillin-susceptible lineage prevalent in North-Eastern Romania before disseminating rapidly throughout Europe. |
| Databáze: | OpenAIRE |
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