| Autor: |
Michele R, Castro, Graciela M, Dias, Tiago S, Salles, Nubia M, Cabral, Danielly C O, Mariano, Hadassa L, Oliveira, Eliana S F W, Abdelhay, Renata, Binato, Bianca C, Neves |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Current genetics. 68(2) |
| ISSN: |
1432-0983 |
| Popis: |
Pseudomonas aeruginosa is an opportunistic pathogen and an important model organism for the study of bacterial group behaviors, including cell motility and biofilm formation. Rhamnolipids play a pivotal role in biofilm formation and motility phenotypes in P. aeruginosa, possibly acting as wetting agents and mediating chemotactic stimuli. However, no biochemical mechanism or gene regulatory network has been investigated in regard to rhamnolipids' modulation of those group behaviors. Using DNA microarrays, we investigated the transcriptomic profiles in the stationary phase of growth of wild-type P. aeruginosa PAO1 and a rhlA-mutant strain, unable to produce rhamnolipids. A total of 134 genes were differentially expressed, comprising different functional categories, indicating a significant physiological difference between the rhamnolipid-producing and -non-producing strains. Interestingly, several flagellar genes are repressed in the mutant strain, which directly relates to the inability of the rhlA-minus strain to develop a swarming-motility phenotype. Supplementation with exogenous rhamnolipids has partially restored flagellar gene expression in the mutant strain. Our results show significant evidence that rhamnolipids, the major biosynthetic products of rhlABC pathway, seem to modulate gene expression in P. aeruginosa. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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