Three-dimensional arrangement of short DNA oligonucleotides at surfaces via the synthesis of DNA-branched polyacrylamide brushes by SI-ATRP

Autor:	Olivier Y F, Henry, Ahmed D, Mehdi, Sinead, Kirwan, Josep Luis Acero, Sanchez, Ciara K, O'Sullivan
Rok vydání:	2011
Předmět:	BRCA1 Protein Acrylic Resins Oligonucleotides Humans Breast Neoplasms Female DNA Exons Genetic Therapy Polymerization
Zdroj:	Macromolecular rapid communications. 32(18)
ISSN:	1521-3927
Popis:	Short DNA oligonucleotide branches are incorporated into acrylamide brushes via surface initiated atom transfer radical polymerization in an attempt to increase DNA surface density by building three-dimensional molecular architectures. ATR-FTIR as well as hybridization studies followed by SPR confirm the incorporation of the DNA sequences into the polymer backbone. MALDI-TOF analysis further suggests that six acrylamide monomer units are typically separating DNA branches present on a single brushes approximately 26 units long. This new approach offers a promising alternative to SAM-based nucleic acid and aptamer sensors and could enable the realization of more complex soft materials of controlled architecture capable of both recognition and signaling by including additional optically or electrochemically active moieties.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::1b05d5e63348ac1a2605cfd771bf9a22 https://pubmed.ncbi.nlm.nih.gov/21800393 Zobrazit plný text záznamu Plný text