| Autor: |
Xiao-chun, Lu, Zhen-hua, Yuan, Jian-qiang, Peng, Xin, Ma, Xiao-bing, Wu, Xiao-ying, Li |
| Rok vydání: |
2004 |
| Předmět: |
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| Zdroj: |
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology. 17(4) |
| ISSN: |
1003-9279 |
| Popis: |
Constructing a plasmid containing tRNAVal promoter to express shRNA which mediates RNA interference.A tRNAVal gene was amplified from human genomic DNA by PCR and replaced the last several bases of 3' end by a linker. The tRNAVal promoter after artificial mutation followed a shRNA sequence to luciferase was cloned into pUC18, Puc-tRNAVal, lucRi Cotransfected with pMAMneoLuc into BHK-21 cell to detect the effect of luciferase expression.pUC-tRNAVallucRi suppressed the luciferase expression from pMAMneoLuc by 97.9%-9.5%.The results showed that the tRNAVal shRNA plasmid could efficiently suppress luciferase expression in BHK-21. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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