| Autor: |
Xiao-qing, Li, Wen-fang, Huang, Hua, Liu, Yong-chang, Yang, Dai-wen, Xiao, Hui, Yan, Chun-li, Luo |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 28(6) |
| ISSN: |
1007-8738 |
| Popis: |
To establish a protein fingerprint database of Salmonella paratyphi A by surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS).Thirty-six clinical bacterial isolates and 96 control bacteria isolates were collected and identified using 16S rDNA sequencing. Bacterial proteins were detected by SELDI-TOF-MS, and all protein fingerprints were analyzed by ProteinChip and Biomarker Wizard software. The analysis results were used to set up a classification tree model by means of BioMarker Patterns software. At the same time, the data were tested by a blinded validation.In the range of M(r); 3 000-20 000, we obtained 104 protein peaks, of which 90 were of statistical significance (P0.01). A protein peak with mass-to-charge ratio(M/Z) 10 061.7 was chosen to establish the classification tree model of Salmonella paratyphi A, and the sensitivity and specificity of Salmonella paratyphi A diagnosis was 100% as shown by the blinded validation.The classification tree model of Salmonella paratyphi A can be not only established using SELDI-TOF-MS technology, but also used for the rapid identification of Salmonella paratyphi A. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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