[A normalized cDNA library from human erythroleukemia cells]

Autor:	A T, Puzyrev, K, Chroniary, N K, Moschonas
Rok vydání:	1995
Předmět:	Genomic Library DNA Complementary Base Sequence Genome Human Molecular Sequence Data Tumor Cells Cultured Humans Leukemia Erythroblastic Acute RNA Messenger Cloning Molecular Chromatography Ion Exchange Nucleic Acid Denaturation DNA Primers
Zdroj:	Molekuliarnaia biologiia. 29(1)
ISSN:	0026-8984
Popis:	Normalized cDNA library from human erythroleukemia cells has been constructed. For equalizing frequencies of different cDNAs denaturation was carried out followed by partial reassociation. Single-stranded cDNAs separated by hydroxyapatite chromatography were transformed into double-stranded form by PCR and cloned in lambda gt11. Frequencies of 10 control nucleotide sequences were estimated. After normalization frequencies of the abundant sequences decreased. The normalized cDNA library may be used for search of the clones corresponding to the rare mRNAs and for human genome mapping.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::1a294eeb1be7cc7fbc17b4b85545aa7d https://pubmed.ncbi.nlm.nih.gov/7723768 Zobrazit plný text záznamu