| Autor: |
Itzia S, Gómez-Alonso, Ilse D, Estrada-Alemán, Sergio, Martínez-García, Humberto, Peralta, Erika T, Quintana, Claudia, Guerrero-Barajas, Cipriano, Chávez-Cabrera, Sandra, Rodríguez-Martínez, Mario E, Cancino-Diaz, Juan C, Cancino-Diaz |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Archives of microbiology. 204(5) |
| ISSN: |
1432-072X |
| Popis: |
The Staphylococcus aureus SdrG protein is glycosylated by SdgA and SdgB for protection against its degradation by the neutrophil cathepsin G. So far, there is no information about the role of Staphylococcus epidermidis SdgA or SdgB in biofilm-forming; therefore, the focus of this work was to determine the distribution and expression of the sdrG, sdgA and sdgB genes in S. epidermidis under in vitro and in vivo biofilm conditions. The frequencies of the sdrG, sdgA and sdgB genes were evaluated by PCR in a collection of 75 isolates. Isolates were grown in dynamic (non-biofilm-forming) or static (biofilm-forming) conditions. The expression of sdrG, sdgA and sdgB was determined by RT-qPCR in cells grown under dynamic conditions (CGDC), as well as in planktonic and sessile cells from a biofilm and cells adhered to a catheter implanted in Balb/c mice. The sdrG and sdgB genes were detected in 100% of isolates, while the sdgA gene was detected in 71% of the sample (p 0.001). CGDC did not express sdrG, sdgA and sdgB mRNAs. Planktonic and sessile cells expressed sdrG and sdgB, and the same was observed in cells adhered to the catheter. In particular, one isolate, capable of inducing a biofilm under treatment with cathepsin G, expressed sdrG and sdgB in planktonic and sessile cells and cells adhering to the catheter. This suggests that bacteria require biofilm conditions as an important factor for the transcription of the sdgA, sdgB and sdrG genes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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