Human papillomavirus detection in paraffin-embedded cervical carcinomas and metastases of the carcinomas by the polymerase chain reaction
| Autor: | E C, Claas, W J, Melchers, H C, van der Linden, J, Lindeman, W G, Quint |
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| Rok vydání: | 1989 |
| Předmět: |
Lymphoma
Histological Techniques Gene Amplification virus diseases Nucleic Acid Hybridization Uterine Cervical Neoplasms DNA-Directed DNA Polymerase Polymerase Chain Reaction female genital diseases and pregnancy complications Blotting Southern Paraffin Lymphatic Metastasis DNA Viral Humans Female Papillomaviridae Research Article |
| Zdroj: | The American journal of pathology. 135(4) |
| ISSN: | 0002-9440 |
| Popis: | The polymerase chain reaction (PCR) is used for human papillomavirus (HPV) detection in paraffin-embedded tissue. The specificity of the reaction is unaffected by the method of fixation used before embedding into paraffin. Five HPV 16, 18, 31, and 33 DNA in situ hybridization (DISH)-negative cervical carcinomas were subjected to the PCR. In two patients, HPV 16 DNA could be detected in the cervical squamous cell carcinomas and also in their lymph node metastases. One patient with an adeno-carcinoma of the cervix was found positive for HPV-18. A lymph node of this patient was HPV 18 positive as well. In the tumors of the remaining two patients, no HPV 16, 18, or 33 DNA was detected by the PCR. Both negative patients had cervical squamous cell carcinomas. One had a bladder metastasis, whereas the other had a lymph node metastasis and an additional distant metastasis in the lung. HPV DNA positivity in cervical carcinomas correlated with HPV prevalence in the metastases. This relationship can be of use for diagnostic purposes in the pathologic analysis of metastases. |
| Databáze: | OpenAIRE |
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