| Autor: |
Dimitrios, Evangelopoulos, Carolyn M, Shoen, Isobella, Honeyborne, Simon, Clark, Ann, Williams, Galina V, Mukamolova, Michael H, Cynamon, Timothy D, McHugh |
| Rok vydání: |
2021 |
| Zdroj: |
Microorganisms. 10(2) |
| ISSN: |
2076-2607 |
| Popis: |
The turnaround times for phenotypic tests used to monitor the bacterial load ofWe report the adaptation of our robust, culture-free assay utilising 16S ribosomal RNA, developed for sputum, to enumerate the number of bacteria present in animal tissues as a tool to improve the read-outs in preclinical drug efficacy studies.Initial assay adaptation was performed using naïve mouse lungs spiked with known quantities ofWhen tested on lung tissues derived from infected mice, the MBL assay produced comparable results to the bacterial counts in solid culture (colony forming units: CFU). Notably, under specific drug treatments, the MBL assay was able to detect a significantly higher number ofThe MBL assay can enumerate the bacterial load in animal tissues in real time without the need to wait for extended periods for cultures to grow. The readout correlates well with CFUs. Importantly, we have shown that the MBL is able to measure specific populations of bacteria not cultured on solid agar. The adaptation of this assay for preclinical studies has the potential to decrease the readout time of data acquisition from animal experiments and could represent a valuable tool for tuberculosis drug discovery and development. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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