FAT1 modulates EMT and stemness genes expression in hypoxic glioblastoma

Autor:	Chitrangda, Srivastava, Khushboo, Irshad, Bhawana, Dikshit, Parthaprasad, Chattopadhyay, Chitra, Sarkar, Deepak Kumar, Gupta, Subrata, Sinha, Kunzang, Chosdol
Rok vydání:	2017
Předmět:	Gene Expression Regulation Neoplastic Epithelial-Mesenchymal Transition Brain Neoplasms Cell Line Tumor Gene Knockdown Techniques Neoplastic Stem Cells Tumor Cells Cultured Humans RNA Messenger Cadherins Glioblastoma Hypoxia-Inducible Factor 1 alpha Subunit Cell Hypoxia
Zdroj:	International journal of cancer. 142(4)
ISSN:	1097-0215
Popis:	Glioblastoma (GBM) is characterized by the presence of hypoxia, stemness and local invasiveness. We have earlier demonstrated that FAT1 promotes invasiveness, inflammation and upregulates HIF-1α expression and its signaling in hypoxic GBM. Here, we have identified the role of FAT1 in regulating EMT (epithelial-mesenchymal transition) and stemness characteristics in GBM. The expression of FAT1, EMT (Snail/LOX/Vimentin/N-cad), stemness (SOX2/OCT4/Nestin/REST) and hypoxia markers (HIF-1α/VEGF/PGK1/CA9) was upregulated in ≥39% of GBM tumors (n = 31) with significant positive correlation (p ≤ 0.05) of the expression of FAT1 with LOX/Vimentin/SOX2/HIF-1α/PGK1/VEGF/CA9. Furthermore, positive correlation (p ≤ 0.01) of FAT1 with Vimentin/N-cad/SOX2/REST/HIF-1α has been observed in TCGA GBM-dataset (n = 430). Analysis of cells (U87MG/A172) exposed to severe hypoxia (0.2%O
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::1966baac9667b091fb355410764f4e24 https://pubmed.ncbi.nlm.nih.gov/28994107 Zobrazit plný text záznamu Plný text