| Autor: |
Phillip, Kyriakakis, Marianne, Catanho, Nicole, Hoffner, Walter, Thavarajah, Vincent J, Hu, Syh-Shiuan, Chao, Athena, Hsu, Vivian, Pham, Ladan, Naghavian, Lara E, Dozier, Gentry N, Patrick, Todd P, Coleman |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
ACS Synthetic Biology |
| ISSN: |
2161-5063 |
| Popis: |
Transplanting metabolic reactions from one species into another has many uses as a research tool with applications ranging from optogenetics to crop production. Ferredoxin (Fd), the enzyme that most often supplies electrons to these reactions, is often overlooked when transplanting enzymes from one species to another because most cells already contain endogenous Fd. However, we have shown that the production of chromophores used in Phytochrome B (PhyB) optogenetics is greatly enhanced in mammalian cells by expressing bacterial and plant Fds with ferredoxin-NADP+ reductases (FNR). We delineated the rate limiting factors and found that the main metabolic precursor, heme, was not the primary limiting factor for producing either the cyanobacterial or plant chromophores, phycocyanobilin or phytochromobilin, respectively. In fact, Fd is limiting, followed by Fd+FNR and finally heme. Using these findings, we optimized the PCB production system and combined it with a tissue penetrating red/far-red sensing PhyB optogenetic gene switch in animal cells. We further characterized this system in several mammalian cell lines using red and far-red light. Importantly, we found that the light-switchable gene system remains active for several hours upon illumination, even with a short light pulse, and requires very small amounts of light for maximal activation. Boosting chromophore production by matching metabolic pathways with specific ferredoxin systems will enable the unparalleled use of the many PhyB optogenetic tools and has broader implications for optimizing synthetic metabolic pathways. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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