| Autor: |
B, Zhou, M, Ensell, Y, Zhou, U, Nair, J, Glickstein, M H, Kermany, Q, Cai, C, Cai, W, Liu, Y-P, Deng, A, Kakigi, M, Barbieri, M, Mora, S, Kanangat, T J, Yoo |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Allergy. 67(2) |
| ISSN: |
1398-9995 |
| Popis: |
One-fourth of the US population is sensitized to the German cockroach. Primary German cockroach allergen Bla g 1 is detected in 63% of homes and 52% of childcare facilities in the United States. No effective treatment or vaccination strategies are yet available.We evaluated the prophylactic and therapeutic efficacy of a plasmid DNA-mediated vaccination using the Bla g 1 gene in a mouse model of allergic inflammatory airway disease.A plasmid DNA vector coding for the Bla g 1 allergen controlled by cytomegalovirus promoter was constructed. To estimate the protective efficacy, BALB/c mice were given three injections of plasmid DNA-Bla g 1 prior to sensitization with two priming doses of recombinant Bla g 1 (rBla g 1) antigens, followed by nebulized rBla g 1 challenge. In the therapeutic approach, sensitization was followed by administering Bla g 1 DNA vaccine.Bla g 1 vaccination significantly reduced allergen-induced airway inflammation, even after mice were presensitized and a Th2-dominant response was established. The Bla g 1 vaccination significantly reduced total inflammatory cell infiltrate, eosinophilia, secretion of Th2 cytokines IL-4 and IL-5 in bronchoalveolar lavage fluid, allergen-induced inflammatory infiltrates in the lungs, and Bla g 1-specific IgE in serum upon challenge with rBla g 1. Importantly, Bla g 1 DNA vaccination was able to induce IL-10-secreting regulatory T cells that could suppress the allergen-specific Th2 cells.DNA vaccination showed protective and therapeutic efficacy against a clinically relevant allergen Bla g 1. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
Plný text