| Autor: |
Emily Y, Smith, James L, Resnick |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 925 |
| ISSN: |
1940-6029 |
| Popis: |
Primordial germ cells (PGCs) play essential roles in both reproduction and development. In this chapter, we describe a method used in our laboratory for the immunopurification of PGCs from the mouse embryo. After dissection and disruption of the fetal gonad, PGCs are identified by a monoclonal antibody recognizing an epitope characteristic of pluripotent stem cells. After reaction with a paramagnetic bead-linked secondary antibody, the cell mixture is applied to a strong magnetic field. PGCs are recovered by release from the magnetic field. Purity is assessed by the alkaline phosphatase activity inherent to PGCs. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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