[Cloning of terminal fragments of the avian adenovirus CELO genome and isolation of a recombinant plasmid carrying the viral oncogene]

Autor: V A, Krugliak, T A, Akopian, B S, Naroditskiĭ, I M, Kartashova, V Ia, Shevliagin
Rok vydání: 1988
Předmět:
Zdroj: Molekuliarnaia genetika, mikrobiologiia i virusologiia. (5)
ISSN: 0208-0613
Popis: The terminal fragment of avian adenovirus CELO has been cloned in a plasmid vector. The obtained recombinant plasmid pCBE1 carries the terminal BamHI-E fragment of CELO DNA. Transfection of a nonpermissive culture of Rat2 cell line by the plasmid DNA results in formation of transformation focuses. The cloned BamHI-E fragment of CELO DNA is concluded to contain the viral oncogene. Thus, the CELO genome region deriving the BamHI-E fragment is "left".
Databáze: OpenAIRE