Age-related alterations in pre-synaptic and receptor-mediated cholinergic functions in rat brain
| Autor: | R L, Büyükuysal, I H, Ulus, B K, Kiran |
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| Rok vydání: | 1998 |
| Předmět: |
Cerebral Cortex
Male Aging Physostigmine Brain Muscarinic Antagonists In Vitro Techniques Phosphatidylinositols Hippocampus Receptors Muscarinic Acetylcholine Corpus Striatum Rats Quinuclidinyl Benzilate Rats Sprague-Dawley Kinetics Membrane Lipids Organ Specificity Synapses Animals Carbachol Phospholipids |
| Zdroj: | Neurochemical research. 23(5) |
| ISSN: | 0364-3190 |
| Popis: | Fractional [3H]acetylcholine (ACh) release and regulation of release process by muscarinic receptors were studied in corpus striatum of young and aged rat brains. [3H] Quinuclidinyl benzilate (QNB) binding and carbachol stimulated phosphoinositide turnover, on the other hand, were compared in striatal, hippocampal and cortical tissues. High potassium (10 mM)-induced fractional [3H]ACh release from striatal slices was reduced by aging. Although inhibition of acetylcholinesterase with eserine (20 microM) significantly decreased stimulation-induced fractional [3H]ACh release in two groups of rats, this inhibition slightly lessened with aging. Incubation of striatal slices with muscarinic antagonists reversed eserine-induced inhibition in fractional [3H]ACh release with a similar order of potency (atropine = 4-DAMPAF-DX 116pirenzepine) in young and aged rat striatum, but age-induced difference in stimulated ACh release was not abolish by muscarinic antagonists. These results suggested that fractional [3H]ACh release from striatum of both age groups is modulated mainly by M3 muscarinic receptor subtype. Although both muscarinic receptor density and labeling of inositol lipids with [myo-3H]inositol decreased with aging, carbachol-stimulated [3H]myo inositol-1-fosfat (IP1) accumulation was found similar in striatal, cortical and hippocampal slices. |
| Databáze: | OpenAIRE |
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