Sperm antigen 6 is the murine homologue of the Chlamydomonas reinhardtii central apparatus protein encoded by the PF16 locus
| Autor: | R, Sapiro, L M, Tarantino, F, Velazquez, M, Kiriakidou, N B, Hecht, M, Bucan, J F, Strauss |
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| Rok vydání: | 2000 |
| Předmět: |
Male
DNA Complementary Base Sequence Sequence Homology Amino Acid Algal Proteins Blotting Western Molecular Sequence Data Chromosome Mapping Transfection Spermatozoa Mice Flagella Organ Specificity Sequence Analysis Protein COS Cells Testis Microtubule Proteins Animals Amino Acid Sequence Chlamydomonas reinhardtii |
| Zdroj: | Biology of reproduction. 62(3) |
| ISSN: | 0006-3363 |
| Popis: | A cDNA encoding sperm antigen 6 (Spag6), the murine homologue of the Chlamydomonas reinhardtii PF16 protein-a component of the flagella central apparatus-was isolated from a mouse testis cDNA library. The cDNA sequence predicted a 55.3-kDa polypeptide containing 8 contiguous armadillo repeats with 65% amino acid sequence identity and 81% similarity to the Chlamydomonas PF1 protein. An antipeptide antibody generated against a C-terminal sequence recognized a 55-kDa protein in sperm extracts and localized Spag6 to the principal piece of permeabilized mouse sperm tails. When expressed in COS-1 cells, Spag6 colocalized with microtubules. The Spag6 gene was found to be highly expressed in testis and was mapped using the T31 radiation hybrid panel to mouse chromosome 16. Mutations in the Chlamydomonas PF16 gene cause flagellar paralysis. The presence of a highly conserved mammalian PF16 homologue (Spag6) raises the possibility that Spag6 plays an important role in sperm flagellar function. |
| Databáze: | OpenAIRE |
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