Pinocembrin Ameliorates Cognitive Impairment Induced by Vascular Dementia: Contribution of Reelin-dab1 Signaling Pathway
| Autor: | Ze-Chun, Kang, Hai-Gang, Wang, Yu-Lin, Yang, Xiao-Yue, Zhao, Qi-Meng, Zhou, Ying-Lin, Yang, Jing-Yu, Yang, Guan-Hua, Du |
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| Rok vydání: | 2020 |
| Předmět: |
Male
signaling pathway Cell Survival hippocampus Cell Adhesion Molecules Neuronal Nerve Tissue Proteins Cell Line Tumor Animals Humans Cognitive Dysfunction RNA Small Interfering Rats Wistar Cells Cultured Adaptor Proteins Signal Transducing Original Research cognitive impairment Extracellular Matrix Proteins Behavior Animal Dose-Response Relationship Drug pinocembrin Dementia Vascular Serine Endopeptidases vascular dementia Reelin Rats Reelin Protein nervous system Flavanones Signal Transduction |
| Zdroj: | Drug Design, Development and Therapy |
| ISSN: | 1177-8881 |
| Popis: | Background As a substrate of apoER2, Reelin has been verified to exert neuroprotection by preventing memory impairment. Pinocembrin is the most abundant natural flavonoid found in propolis, and it has been used to exert neuroprotection, blood–brain barrier protection, anti-oxidation, and inflammation diminishing, both in vitro and in vivo. However, the roles and molecular mechanisms of pinocembrin in neurobehavioral outcomes and neuronal repair after vascular dementia are still under investigation. Purpose To explore the role of pinocembrin in the involvement of the Reelin-dab1 signaling pathway in improving memory impairment, both in cell culture and animals experiments. Material and Methods Behavioral tests were conducted on day 48 to confirm the protection of pinocembrin against cognitive impairment. Cell and molecular biology experiments demonstrated that the Reelin-dab1 pathway mediates the underlying mechanism of cognitive improvement by pinocembrin. Results It was showed that pinocembrin alleviated learning and memory deficits induced by vascular dementia, by inducing the expression of Reelin, apoER2, and p-dab1 in the hippocampus. The expression of Reelin and p-dab1 was both inhibited following Reelin RNA interference in SH-SY5Y prior to oxygen glucose deprivation (OGD) injury, suggesting that Reelin played a core role in pinocembrin’s effect on OGD in vitro. Conclusion Pinocembrin improves the cognition via the Reelin-dab1 signaling pathway. |
| Databáze: | OpenAIRE |
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