P-glycoprotein-associated Cl- currents are activated by cell swelling but do not contribute to cell volume regulation
| Autor: | G A, Altenberg, J W, Deitmer, D C, Glass, L, Reuss |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Intracellular Fluid
Cell Membrane Permeability Membrane Glycoproteins Drug Resistance Breast Neoplasms Transfection Membrane Potentials Kinetics Chlorides Hypotonic Solutions Chloride Channels Electrochemistry Potassium Tumor Cells Cultured Humans Female ATP Binding Cassette Transporter Subfamily B Member 1 Carrier Proteins Cell Size |
| Zdroj: | Cancer research. 54(3) |
| ISSN: | 0008-5472 |
| Popis: | P-glycoprotein-associated Cl- conductance is activated by cell swelling; the ensuing Cl- efflux is thought to contribute to cell volume regulation. We tested this hypothesis in human breast cancer cells transfected with human mdr1 complementary DNA, which display P-glycoprotein-associated, swelling-activated Cl- currents. The Cl- electrochemical driving force favors Cl- efflux, but there was no appreciable Cl- loss or regulatory volume decrease (both assessed with fluorescent dyes) during the exposure to hyposmotic solution. Calculations indicate that the swelling-activated Cl- current is insufficient to cause a significant Cl- efflux. Hence, regulatory volume decrease is not a function of P-glycoprotein. |
| Databáze: | OpenAIRE |
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