| Autor: |
Ying, Wang, Lei-ming, Zhou, Ju-li, Gong, Ming-kui, Zhou, Fang-wei, Shen, Xiao-hong, Zheng, Wei, Zhang, Ming-hua, Zhuang, Wei, Lu, Ping, Zhong, Qi-chao, Pan, Lai-yi, Kang |
| Rok vydání: |
2007 |
| Předmět: |
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| Zdroj: |
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 23(9) |
| ISSN: |
1007-8738 |
| Popis: |
To determine the frequency of the CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes from the peripheral blood in the Chinese healthy individuals and provide some useful evidence for clinical research of correlative diseases.From the CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes of peripheral blood in 312 Chinese healthy male and female individuals aged from 8 to 60(five age groups were collected) The expression of transcription factor Foxp3 was detected by triplex immuno fluorescence and the frequency of CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes was determined by flow cytometry.The frequency of CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes in Chinese healthy individuals was (6.55+/-0.11)%, and the frequency differed among age groups(P=0.015) and sex groups(P0.05). CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes specifically express transcription factor Foxp3.The frequency of the CD4(+)CD25(nt/hi)CD127(lo) regulatory T lymphocytes from the peripheral blood in the Chinese healthy individuals has been preliminarily determined which lays the foundation for further clinical research of regulatory T lymphocytes. As a specific cell surface marker, CD25(nt/hi)CD127(lo) can helpful obtain pure CD4(+)CD25(+) regulatory T lymphocytes and suppress the interference of other cells during cell separation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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